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國立嘉義大學
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陳逸然副教授(合聘)

陳逸然 副教授            


一、學歷：
1998-2002 國立台灣大學化學研究所
2002-2006 中央研究院化學研究所博士後研究

二、經歷：
1998-2002 國立台灣大學化學研究所
2002-2006 中央研究院化學研究所博士後研究
2006-2007 國立台灣海洋大學生物科技研究所助理教授
2007-2015 中央研究院農業生物技術研究中心助研究員
2015-迄今 中央研究院農業生物技術研究中心副研究員

三、專業領域：

四、連絡方式（ Tel or e-mail)：

電話：(02) 2787-2050     E-mail：yetran@gate.sinica.edu.tw　　

五、研究方向：

胜荷爾蒙訊息傳遞對植物發育影響之研究

先前研究中指出，高等植物細胞與細胞間之相互作用主要受到小分子親脂性化合物之調控。直到現在，有越來越多研究顯示胜分子也會對植物的發育造成影響，如防禦反應、癒合組織的生長、頂端分生組織的構型組成、自體不和合、根部組織之生長、葉形調控、根瘤發育及組織剝落等。阿拉伯芥基因組的完整定序結果顯示此種植物較他種已定序完成的生物具有超過十倍以上不同的胜傳送分子；相較於人類已知約有近58 種receptor-like kinase (RLK)，植物則可能具有超過400 種的RLK，而阿拉伯芥的RLK 中則包含超過210種lucine rich repeat-receptor likekinases (LRR-RLKs) 的基因；LRR domain 已知主要扮演蛋白質- 蛋白質或蛋白質- 胜間的相互作用。有鑑於此發現，植物目前被預期應含有極大數量的訊息傳遞胜及相對應的受器並在植物發育過程中扮演極重要的角色。這些胜荷爾蒙透過細胞膜上之LRR-RLKs 的相互作用，活化下游之訊息傳導路徑。然而目前僅有少量的植物訊息胜被發現，有鑑於此，我們將整合並發展找尋參與植物發育上的重要訊息傳遞胜以及perceptive receptors 之研究平台，並結合蛋白質體學技術研究訊息傳遞的路徑，此研究成果將能提供植物胜荷爾蒙功能更詳盡之過程，並且能加以區分動植物間胜訊息傳導路徑的相似處及差異點。藉此訊息傳遞路徑網的建立，幫助我們改善植物對病原的抵抗力、抗壓力及其他發育功能的提升。

建立高效表現蛋白質體學及代謝體學之技術

與疾病相關之生物標記可用來作為研究疾病之形成機制，並以此建立早期診斷標準及後續治療策略的擬定。目前質譜技術的發展已大為提高白質體及代謝體的特性研究。我們期望藉由質譜儀的分析，建立可應用於找尋與疾病相關之生物標記之蛋白質體及代謝體研究技術，並以此技術發展普及且廣泛的疾病相關研究平台，以期最終能作為研究具潛力之治療藥物上一有力工具。

六、期刊著作：

  1. Y. L. Chen, C. Y. Lee, K. T. Cheng, W. H. Chang, R. N. Huang, H. G. Nam and Y. R. Chen* “Quantitative Peptidomics Study Reveals a Wound-Induced Peptide from PR-1 Regulates Immune Signaling in Tomato” Plant Cell, (2014) October 31. (SCI Category: Plant Science, IF:10.125, Category Ranking (excluding review journals):1/194)
  2. T. K.i Huang, C. L. Han, S. I. Lin, Y. J. Chen, Y. C. Tsai, Y. R. Chen, J. W. Chen, W. Y. Lin, P. M. Chen, T. Y. Liu, Y. S. Chen, C. M. Sun, and T. J. Chiou * "Identification of Downstream Components of PHO2 by Quantitative Membrane Proteomics in Arabidopsis Roots" Plant Cell (2013) accepted.(SCI Category: Plant Science, IF(5-year):10.125, Category Ranking (excluding review journals):1/194)
  3. J. H. Weng, M. R. Liang, C. H. Chen, S. K. Tong, T. C. Huang, S. P. Lee, Y. R. Chen, C. T. Chen, and B. Chung "Pregnenolone changes CLIP-170 conformation to promote microtubule polymerization, cell migration, and zebrafish development" Nature Chemical Biology (2013) accepted.
  4. J. Koubek, K. F. Lin, Y. R. Chen, R. P. Cheng, J. J. Huang “Strong anion exchange fast performance liquid chromatography as a versatile tool for preparation and purification of RNA produced by in vitro transcription” RNA (2013) accepted.
  5. H. Y. Lin, C. Y. Shih, H. C. Liu, J. Chang & Y. L. Chen, Y. R. Chen, H. T. Lin, Y. Y. Chang, C. H. Hsu and H. J. Lin “Identification and Characterization of an Extracellular Alkaline Phosphatase in the Marine Diatom Phaeodactylum tricornutum” Mar Biotechnol. 15 (2013) 425. (SCI Category: Medicine, Research & Experimental, IF:1.545, Category Ranking:23/77)
  6. Y. C. Chang, Y. H. Yu, J. Y. Shew, W. J. Lee, J. J. Hwang, Y. H. Chen, Y. R. Chen, P. C. Wei, L. M. Chuang*, W. H. Lee* “Deficiency of NPGPX, an oxidative stress sensor, leads to obesity in mice and human” EMBO Mol. Med., 5 (2013) 1 (SCI Category: Medicine, Research & Experimental, IF:7.795, Category Ranking:9/121)
  7. B. Wiench, Y. R. Chen, M. Paulsen, R. Hamm, S. Schroder, N.S. Yang, and T. Efferth “Integration of different “-omics” technologies identifies inhibition of the IGF1R-Akt-mTOR signaling cascade involved in the cytotoxic effect of shikonin against leukemia cells” Evidence-Based Complementary and Alternative Medicine (2013) accepted. (IF: 4.774)
  8. W. H. Chang, C. Y. Lee, C. Y. Lin; M. C. Chen, W. S. Tzou and Y. R. Chen* “UniQua: A Universal Signal Processor for MS-Based Qualitative and Quantitative Proteomics Applications” Anal. Chem., 85 (2013) 890 (SCI Category: Chemistry, Analytical, IF:5.903, Category Ranking:1/71)
  9. M. Tsednee, Y. W. Mak, Y. R. Chen and K. C. Yeh “A sensitive LC-ESI-TOF-MS method reveals novel phytosiderophores and phytosiderophore–iron complexes in barley” New Philologist, (2012) Jun. 18 (SCI Category: Plant Science, IF:6.645, Category Ranking:6/190)
  10. L. T. Huang, C. C. Wu, L. F. Lai, M. M. Gromiha, C. S.Wang, Y. R. Chen "Data mining application in biomedical informatics for probing into protein stability upon double mutation" Applied Mathematics and Information Sciences, 7(2S) (2013) 567S.
  11. H. C. Tseng, H. W. Kao, M. R. Ho, Y. R. Chen, T. W. Lin, P. C. Lyu and W. C. Lin “Cytoskeleton Network and Cellular Migration Modulated by Nuclear-localized Receptor Tyrosine Kinase ROR1” Anticancer Res., 31, (2011) 4239.
  12. C.J. Chen, W.Y. Chen, M.C. Tseng, and Y. R. Chen* (2011) Tunnel Frit: A Nonmetallic In-Capillary Frit for Nanoflow Ultra High-Performance Liquid Chromatography–Mass SpectrometryApplications. Anal. Chem., DOI: 10.1021/ac202549y
  13. Chen, C.J., Tseng, M.J., Lin, H.J. and Chen, Y.R.* (2010) A Rapid Visualized Indicator for Surfactant Abundance (VISA) in MS-Based Membrane and General Proteomics Application. Anal. Chem., 82, 8283-8290.
  14. W. T. Lin, W. N. Hung, Y. H. Yian, Y. R. Chen Y. J. Chen, T. Y. Sung, W. L. Hsu, K. P. Wu "Multi-Q: a Fully Automated Tool for Multiplexed Protein Quantitation" J. Proteome Res., 5 (2006) 2328.
Print
  1. Y. R. Chen, H. F. Juan, H. C. Huang. H. H. Huang, Y. J. Lee, M. Y. Liao, C. W. Tseng, L. L. Lin, J. Y. Chen, M. J. Wang, J. H. Chen, Y. J. Chen "Quantitative Proteomic and Genomic Profiling Reveals Metastasis-Related Protein Expression Patterns in Gastric Cancer Cells" J. Proteome Res., 5 (2006) 2727.
  2. Y. R. Chen, M. C. Tseng and G. R. Her "Design and performance of a low-flow capillary electrophoresis-electrospray-mass spectrometry interface using an emitter with dual beveled edge" Electrophoresis, 26 (2005) 1376.
  3. W. C. Kao, Y. R. Chen, E. C. Yi, H. Lee, Q. Tian, K. M. Wu, S. F. Tsai, S. S. F. Yu, Y. J. Chen, R. Aebersold and S. I. Chan "Quantitative Proteomic Analysis of Metabolic Regulation by Copper Ions in Methylococcus capsulatus (Bath)" J. Biol. Chem., 279 (2004) 51554.
  4. M. C. Tseng, Y. R. Chen and G. R. Her "A low-makeup beveled tip capillary electrophoresis /electrospray ionization mass spectrometry interface for micellar electrokinetic chromatography and nonvolatile buffer capillary electrophoresis." Anal. Chem., 76 (2004) 6306.
  5. M. C. Tseng, Y. R. Chen and G. R. Her, "A Beveled Tip Sheath Liquid Interface for Capillary Electrophoresis – Electrospray Ionization Mass Spectrometry"Electrophoresis, 25 (2004) 2084.
  6. Y. R. Chen and G. R. Her, "A Simple Method for Fabrication of Silver Coated Sheathless Electrospray Emitter" Rapid. Commum. Mass Spectrom., 17 (2003) 437.
  7. Y. R. Chen, M. C. Tseng, Y. Z. Chang and G. R. Her, " Low-Flow CE/Electrospray Ionization MS Interface for Capillary Zone Electrophoresis, Large-Volume Sample Stacking, and Micellar Electrokinetic Chromatography"Anal. Chem., 75 (2003) 503.
  8. S. T. Hsiao, M. C. Tseng, Y. R. Chen and G. R. Her, "Analysis of Polymer Additives by Matrix Assisted Laser Desorption Ionization / Time of Flight Mass Spectrometer Using Delay Extraction and Collision Induced Dissociation" J. Chin. Chem. Soc. 48 (2001) 1017.
  9. Y. Z. Chang, Y. R. Chen and G. R. Her, "Sheathless Capillary Electrophoresis/Electrospray Mass Spectrometry Using a Carbon-Coated Tapered Fused-Silica Capillary with Beveled Edge." Anal. Chem., 73 (2001) 5083.
  10. Y. R. Chen, D. D. Ju and G. R. Her, "System Peaks of Cyclodextrins in Capillary Electrophoresis" J. High Resol. Chromatogr. 23 (2000) 409.
  11. Y. R. Chen, K. C. Wen and G. R. Her, "Analysis of Coptisine, Berberine and Palmatine in Adulterated Chinese Medicine by Capillary –Electrospray Ion Trap Mass Spectrometry" J. Chromatogr. A, 866.
  12. C. Y. Tsai, Y. R. Chen and G. R. Her, "Analysis of triazine by reversed electroosmotic flow capillary electrophoresis-electrospray mass spectrometry" J. Chromatogr. A, 813 (1998) 379.
  13. Y.L. Chen, C.Y. Lee, K.T. Cheng, W.H. Chang, R.N. Huang, H.G. Nam and Y.R. Chen* “Quantitative Peptidomics Study Reveals a Wound-Induced Peptide from PR-1 Regulates Immune Signaling in Tomato” Plant Cell (2014) October 31.

七、 研討會論文

八、研究計畫：

九、其他：

十、教授課目：