國立嘉義大學入口網應用化學系暨研究所林榮流教授

林榮流特聘教授

一、學歷：

南非開普敦大學微生物學博士

二、經歷：

1991-1995 新竹食品工業研究所研究員

1995-1998 弘光科技大學食品營養系副教授

1998-2002 弘光科技大學食品營養系教授

2002-2004 嘉義大學應用化學系暨研究所副教授

2004-present 嘉義大學應用化學系暨研究所教授

三、專業領域：

生物化學

四、連絡方式（ Tel or e-mail)：

　　052717969;

　　 llin@mail.ncyu.edu.tw

五、研究方向：

　My research interest has been focused on elucidating the mechanism of action and the structure-function relationship of enzymes. One ongoing project is to develop an effective enzymatic method for synthesizing theanine from glutamine and ethylamine by Escherichia coli γ-glutamyltranspeptidase (γ-GGT). Moreover, the conserved residues of recombinant E. coli γ-GGT have been replaced with other amino acids by site-directed mutagenesis. The preliminary results show that some of the residues are involved in the autocatalytical processing of the enzyme. We also cloned and expressed other γ-GGTs from Bacillus stearothermophilus and B. kaustophilus in recombinant E. coli. The overproduced enzymes have great potential for the bioconversion of medical agents. The specific aims include identifying phylogenetic analysis and biochemical characterization of a thermostable dihydropyrimidinase from Bacillus sp. TS-23, analyzing the functions of the conserved amino acids of microbial leucine aminopeptidases (LAPs), and recovering the industrial important enzymes by adsorption-elution on raw starch. The other ongoing project is to study the structure-function relationship of B. stearothermophilus LAP. To study the catalytic mechanism of this enzyme, B. stearothermophilus LAP is subjected to structural analysis by X-ray crystallography and to functional analysis by mutagenesis. By combing the data from both approaches, we hope that the molecular action of LAP can be appreciated in the near future.

六、期刊著作：校務系統研究成果

1. Chi MC, Lo HF, Lin MG, Chen YY, Wang TF*, Lin LL*(2019) Mutational analysis of a highly conserved PLSSMXP sequence in the small subunit of Bacillus licheniformis γ-glutamyltranspeptidase. Biomolecules, 9:508

2. Wang TF, Lo HF*, Chi MC, Lai KL, Lin MG, Lin LL*(2019) Affinity immobilization of a bacterial prolidase onto metal-ion-chelated magnetic nanoparticles for the hydrolysis of organophosphorus compounds. International Journal of Molecular Sciences, 20:3625

3. Chi MC, Lin MG, Huang YF, Chen YY, Wang TF*, Lin LL*(2019) Enzymatic synthesis of L-theanine from L-glutamine and ethylamine by Bacillus licheniformis γ-glutamyltranspeptidase and its mutants specialized in transpeptidase activity. Biocatalysis and Agricultural Biotechnology, 22:101393

4. Lo HF, Chi MC, Lin MG, Lan YG, Wang TF*, Lin LL*(2018) Protective effect of biological osmolytes against heat- and chaotropic agent-induced denaturation of Bacillus licheniformis γ-glutamyltranspeptidase. Journal of Microbiology and Biotechnology, 28: 1457-1466

5. Lee YC, Chi MC, Lin MG, Chen YY, Lin LL*, Wang TF*(2018) Biocatalytic synthesis of γ-glutamyl-L-leucine, a kokumi-imparting dipeptide, by Bacillus licheniformis γ-glutamyltranspeptidase. Food Biotechnology, 32:130-147

6. Wang TF, Chi MC, Lai GL, Lin MG, Chen YY, Lo HF*, Lin LL*(2018) High-level expression and molecular characterization of a recombinant prolidase from Escherichia coli NovaBlue. PeerJ, 6: e5863

7. Chi MC, Lo HF, Lin MG, Chen YY, Lin LL*, Wang TF*(2017) Application of Bacillus licheniformis γ-glutamyltranspeptidase to the biocatalytic synthesis of γ-glutamyl-phenylalanine. Biocatalysis and Agricultural Biotechnology, 10: 278-284

8. Lin LL, Chi MC, Lan EG, Lin MG, Juang TY*, Wang TF*(2017) Facile immobilization of Bacillus licheniformis γ-glutamyltranspeptidase onto graphene oxide nanosheets and its application to the biocatalytic synthesis of γ-L-glutamyl peptides. International Journal of Biological Macromolecules, 117: 1326-1333

9. Chi MC, Lin MG, Chen YY, Lin LL*, Wang TF*(2017) Functional role of the conserved glycine residues, Gly481 and Gly482, of the γ-glutamyltranspeptidase from Bacillus licheniformis. International Journal of Biological Macromolecules, 109: 1182-1188

10. Lin LL, Chi MC, Lan EG, Lin MG, Juang TY*, Wang TF*(2017) Facile immobilization of Bacillus licheniformis γ-glutamyltranspeptidase onto graphene oxide nanosheets and its application to the biocatalytic synthesis of γ-L-glutamyl peptides. International Journal of Biological Macromolecules, 17: S0141-8130

11. Chi MC, Lin MG, Chen YY, Lin LL*, Wang TF*(2017) Functional role of the conserved glycine residues, Gly481 and Gly482, of the γ-glutamyltranspeptidase from Bacillus licheniformis. International Journal of Biological Macromolecules, 109:1182-1188

12. Lin MG, Chi MC, Chen YY, Wang TF, Lo HF*, Lin LL* (2016) Site-directed mutagenesis of a conserved Asn450 residue of Bacillus licheniformis γ-glutamyltranspeptidase. International Journal of Biological Macromolecules 91:416-425.

13. Wang CH, Huang CC, Lin LL, Chen W* (2016) The effect of disulfide bonds on protein folding, unfolding, and misfolding investigated by FT–Raman spectroscopy. Journal of Raman SpectroscopyDOI: 10.1002/jrs.4935.

14. Lo HF, Chen BE, Lin MG, Chi MC, Wang TF*, Lin LL* (2016) Gene expression and molecular characterization of a chaperone protein HtpG from Bacillus licheniformis. International Journal of Biological Macromolecules 85:179-191.

15. Lin MG, Wang TF, Chen YY, Chi MC*, Lin LL* (2016) Effects of organic co-solvents on catalytic activity and structural stability of three different Bacillus enzymes. Journal of the Taiwan Institute of Chemical Engineers 59:126-131.

16. Lin MG, Chi MC, Naveen V, Li YC, Lin LL*, Hsiao CD* (2016) Bacillus licheniformis trehalose-6-phosphate hydrolase structures suggest keys to substrate specificity. Acta Crystallographica D72:59-70.

17. Pica A, Chi MC, D’Ischia M, Chen YY, Lin LL, Merlino A* (2016) The maturation mechanism of γ-glutamyltranspeptidases: insights from the crystal structure of a precursor mimic of the enzyme fromBacillus licheniformis. Biochimica et Biophysica Acta – Proteins and Proteomics 1864:195-203.

18. Chen YY, Lo HF, Wang TF, Lin MG, Lin LL*, Chi MC* (2015) Enzymatic synthesis of γ-L-glutamyl-S-allyl-L-cysteine, a naturally occurring organosulfur compound from garlic, by Bacillus licheniformis γ-glutamyltranspeptidase. Enzyme and Microbial Technology 75-76:18-24.

19. Liao HF*, Wu DR, Dai JL, Chi MC, Lin LL* (2015) Immunomodulatory activity of the polysaccharide-rich extract from edible cyanobacterium Nostoc commune. Medical Science 3(4):112-123.

20. Chen JH, Chi MC, Lin MG, Lin LL*, Wang TF (2015) Beneficial effect of sugar osmolytes on the refolding of guanidine hydrochloride-denatured trehalose-6-phosphate hydrolase from Bacillus licheniformis.BioMed Research International Article ID 806847.

21. Lin MG, Chen BE, Chi MC, Wang TF, Lo HF, Lin LL* (2015) Tryptophan substitution at three specific phenylalanine locations of Bacillus licheniformis nucleotide exchange factor GrpE: structural and functional effects. International Journal of Biological Macromolecules 72:724-731.

22. Chi MC, Lo YH, Chen YY, Lin LL*, Merlino A* (2014) γ-Glutamyl transpeptidase architecture: effect of extra sequence deletion on autoprocessing, structure and stability of the protein from Bacillus licheniformis. Biochimica et Biophysica Acta – Proteins and Proteomics 1844:2290-2297.

23. Lin LL, Chen YY, Chi MC, Merlino A* (2014) Low resolution X-ray structure of γ-glutamyltranspeptidase from Bacillus licheniformis: opened active site cleft and a cluster of acid residues potentially involved in the recognition of a metal ion. Biochimica et Biophysica Acta - Proteins and Proteomics 1844:1523-1529.

24. Juang TY*, Kan SJ, Chen YY, Tsai YL, Lin MG, Lin LL* (2014) Preparation of surface-functionized hyperbranched poly(amido acids) magnetic nanocarriers for the covalent immobilization of a bacterialγ-glutamyltranspeptidase. Molecules 19:4997-5012.

25. Ong PL, Chuang TT, Wang TF, Lin LL* (2014) Identification of critical amino acid residues for chloride binding of Bacillus licheniformis trehalose-6-phosphate hydrolase. Biologia 69(1):1-9.

26. Wang TF, Lin MG, Lo HF, Chi MC, Lin LL* (2014) Biophysical characterization of a recombinant aminopeptidase II from the thermophilic bacterium Bacillus stearothermophilus. Journal of Biological Physics 40:25-40.

27. Chi MC, Lin MG, Wang TF, Lo HF, Lin LL* (2013) Influence of asparagine mutations on enzymatic activity and thermostability of a recombinant α-amylase from alkaliphilic Bacillus sp. strain TS-23.Journal of Enzyme Research 4:46-49.

28. Chen YY, Tsai MG, Chi MC, Wang TF*, Lin LL* (2013) Covalent immobilization of Bacillus licheniformis γ-glutamyltranspeptidase on aldehyde-functionalized magnetic nanoparticles. International Journal of Molecular Sciences 14:4613-4628.

29. Lin LL, Merlino A* (2013) Heterogeneous nucleation helps the search for initial crystallization conditions of γ-glutamyl transpeptidase from Bacillus licheniformis. Acta Crystallographica F69:669-672.

30. Ong PL, Chuang TT, Wang TF, Lin LL*. 2013. Implications of amino acid residues Arg201 and Asn327 on chloride binding of trehalose-6-phosphate hydrolase, TreA, from Bacillus licheniformis. Biologia(Under review)

31. Lo HF*, Chen YZ, Lin LL*. 2013. Simultaneous purification and immobilization of histidine-taggedBacillus licheniformis aldehyde dehydrogenase. Journal of Pure and Applied Microbiology (accepted for publication)

32. Chen BE, Lin MG, Lo HF, Wang TF, Chi MC*, Lin LL*. 2013. Introduction of a unique tryptophan residue into various positions of Bacillus licheniformis DnaK. International Journal of Biological Macromolecules 52:231-243.

33. Chi MC, Chen YY, Lo HF, Lin LL*. 2012. Experimental evidence for the involvement of amino acid residue Glu398 in the autocatalytic processing of Bacillus licheniformis γ-glutamyltranspeptidase. FEBS Open Bio 2:298-304.

34. Hu HY, Yang JC, Chen JH, Chi MC*, Lin LL*. 2012. Enzymatic characterization of Bacillus licheniformis γ-glutamyltranspeptidase fused to N-terminally truncated forms of Bacillus sp. TS-23 α-amylase. Enzyme and Microbial Technology 50:86-94.

35. Lee YC*, Lin DT, Chen HL, Lo HF, Hu HY, NW Hsiao, Lin LL*. 2012. Characterization of glycine substitution mutations within the NAD⁺-binding site of Bacillus licheniformis aldehyde dehydrogenase.Protein & Peptide Letters 19: 1183-1193.

36. Chen YH, Chi MC, Wang TF, Chen JC, Lin LL*. 2012. Preparation of magnetic nanoparticles and their use for immobilization of C-terminally lysine-tagged Bacillus sp. TS-23 α-amylase. Applied Biochemistry and Biotechnology 166:1711-1722.

37. Chuang TT, Ong PL, Wang TF, Huang HB, Chi MC, Lin LL*. 2012. Molecular characterization of a novel trehalose-6-phosphate hydrolase, TreA, from Bacillus licheniformis. International Journal of Biological Macromolecules 50:459-470.

38. Chi MC, Wu TJ, Chen HL, Lo HF*, Lin LL*. 2012. Counteraction of temperature- and chemical-induced denaturation of a recombinant Bacillus sp. TS-23 α-amylase by sorbitol. Journal of Industrial Microbiology and Biotechnology 39: 1779-1788.

39. Lee YC, Lin DT, Ong PL, Chen HL, Lo HF*, Lin LL*. 2011. Contribution of conserved Glu255 and Cys289 residues to catalytic activity of a recombinant aldehyde dehydrogenase from Bacillus licheniformis. Biochemistry-Moscow 76:1233-1241.

40. Lo HF, Su JY, Chen HL, Chen JC, Lin LL*. 2011. Biophysical studies of a NAD(P)⁺-dependent aldehyde dehydrogenase from Bacillus licheniformis. European Biophysics Journal 40:1131-1142.

41. Hung CP, Yang JC, Chen JH, Chi MC, Lin LL*. 2011. Unfolding analysis of the mature and unprocessed forms of Bacillus licheniformis γ-glutamyltranspeptidase. Journal of Biological Physics 37:463-475.

42. Chen HL, Lo HF*, Chang CT, Lin LL*. 2011. Substrate specificity and kinetic characterization of a recombinant dipeptidyl carboxypeptidase from Escherichia coli novablue. Research Journal of Microbiology 6(4):410-417

43. Yang JC, Liang WC, Chen YY, Chi MC, Lo HF, Chen HL, Lin LL*. 2011. Biophysical characterization of Bacillus licheniformis and Escherichia coli γ-glutamyltranspeptidases: a comparative analysis.International Journal of Biological Macromolecules 48:414-422.

44. Lin MG, Liang WC, Chen BE, Chou WM, Lin LL*. 2011. Involvement of residues Asp8, Asn13, Glu145, Asp168, and Thr173 in the chaperone activity of a recombinant DnaK from Bacillus licheniformis. Journal of Molecular Microbiology and Biotechnology 20:29-42.

45. Chi MC, Wu DR, Chuang TT, Chen HL, Lo HF, Lin LL*. 2010. Biophysical characterization of a recombinant α-amylase from thermophilic Bacillus sp.. TS-23. The Protein Journal 29:572-582. (SCI)

46. Tsai MY, Chang WH, Liang JY, Lin LL, Chang GC, Chang HP*. 2010. Essential covalent linkage between the chymotrypsin-like domain and the extra domain of the SARS-CoV main protease. Journal of Biochemistry (Tokyo) 149(3):349-358.

47. Chen YH, Chuang LY, Lo HF, Lin LL*, Chi MC*. 2010. Mutational analysis of the proposed calcium-binding aspartates of a truncated α-amylase fromBacillus sp. strain TS-23. Annals of Microbiology 60(2):307-315.

48. Lin MG, Chen BE, Liang WC, Chou WM, Kuo LY, Lin LL*. 2010. Site-saturation mutagenesis of leucine 134 of Bacillus licheniformis nucleotide exchange factor GrpE reveals the importance of this residue to the co-chaperone activity. The Protein Journal 29:365-372.

49. Huang CL, Cheng WC, Yang JC, Chi MC, Chen JH, Lin HP, Lin LL*. 2010. Preparation of carboxylated magnetic particles for the efficient immobilization of C-terminally lysine-tagged Bacillus stearothermophilus aminopeptidase II. Journal of Industrial Microbiology and Biotechnology 37:717-725.

50. Chi MC, Chen AH, Wu DR, Lo HF, Lin LL*. 2010. Engineering of a truncated α-amylase of Bacillus sp. strain TS-23 for the simultaneous improvement of thermal and oxidative stabilities. Journal of Bioscience and Bioengineering 109:531-538.

51. Chang HP, Liang WC, Lyu RC, Chi MC, Wang ZF, Su KL, Hung HC, Lin LL*. 2010. Effects of C-terminal truncation on the autocatalytic processing of a recombinant γ-glutamyltranspeptidase from Bacillus licheniformis. Biochemistry-Moscow 75(7):919-929.

52. Chi MC, Chang HP, Chang GG, Wang TF, Huang HB, Lin LL*. 2010. Biophysical properties of a recombinant leucyl aminopeptidase from Bacillus kaustophilus. Biochemistry-Moscow 75(5):642-647.

七、研討會論文 ：

1. Chi MC, Chen YY, Lin LL. 2013. Glutamate 398 is crucial for the autocatalytic processing of Bacillus licheniformis γ-glutamyltranspeptidase. International Conference on Life Science & Biological Engineering (LS&BE 2013), Tokyo, Japan.

2. Chen BE, Wu DJ, Lin LL. 2013. Biochemical characteristics and pH-induced conformational changes of high temperature protein G (HtpG/Hsp90) from Bacillus licheniformis. International Conference on Life Science & Biological Engineering (LS&BE 2013), Tokyo, Japan.

3. Wu DJ, Lin LL, Dai JL, Liao HF. 2013 Bioactivities and immunomodulatory activity of Nostoc commune. International Conference on Life Science & Biological Engineering (LS&BE 2013) , Tokyo, Japan.

4. Lin DT, Lee YC, Lo HF, Lin LL. 2012. Exploration of the glycine residues essential for NAD⁺-binding of Bacillus licheniformis aldehyde dehydrogenase. Abstracts of 3^rd International Conference on Food Engineering and Biotechnology, Kuala Lumpur, Malaysia.

5. Chi MC, Lin LL. 2012. Influence of osmolytes on the structure and enzymatic activity of a recombinant α-amylase from Bacillus sp. TS-23. Abstracts of 12th Annual Meeting of the protein Science Society of Japan, Nagoya, Japan.

6. Chuang ZT, Ong PL, Lin LL. 2012. Biochemical and biophysical characterization of a trahalose-6-phosphate hydrolase form Bacillus licheniformis. Abstracts of 12th Annual Meeting of the protein Science Society of Japan, Nagoya, Japan.

7. Lin LL, Su JY. 2011. Biophysical characterization of a (NAD(P)⁺-dependent aldehyde dehydrogenase from Bacillus licheniformis. Abstracts (P03.82) of 36^th FEBS Congress, Torino, Italy.

8. Chi MC, Wu DJ, Lin LL. 2011. Biophysical studies of a truncated α-amylase from thermophilic Bacillus sp. strain TS-23. Abstracts (1016) of IX European Symposium Protein of the Protein Society, Stockholm, Sweden.

9. Lo HF, Chen HL, Lin LL. 2011. Immobilization of C-terminally lysine-tagged α-amylase onto adipic acid-modified magnetic nanoparticles. Abstracts of the 12^th ASEAN Food Conference, Bangkok, Thailand.

10. Chi MC, Chen YH, Lin LL. 2010. Engineering of Bacillus sp. strain TS-23 α-amylase for improving its thermal and oxidative stabilities. Abstracts (E5.13) of 35^th FEBS Congress, Gothenburg, Sweden.

11. Lin LL, Lin MG, Chen BE, Liang WC, Chi MC. 2010. Importance of leucine 134 to the co-chaperone activity of Bacillus licheniformis nucleotide exchange factor GrpE. Abstracts (E4.06) of 35^th FEBS Congress, Gothenburg, Sweden.

12. Lin LL, Lin MG, Liang WC, Chi MC. 2009. Role of the C-terminal region of a recombinant DnaK from Bacillus licheniformis as evaluated by a serial deletion. Abstracts of 34^th FEBS Congress, Prague, Czech Republic.

13. Chi MC, Liang WC, Lin LL, Huang HB. 2009. Characterization and C-terminally truncational analysis of a 70-kDa molecular chaperone, DnaK, from the industrial bacterium Bacillus licheniformis. Abstracts of Experimental Biology 2009, New Orleans, Louisiana, USA.

14. Lin LL, Wu CL, Yang JC, Chen YP, Lo HF. 2008. Characterization of lysine-tagged Bacillus stearothermophilus leucine aminopeptidase II immobilized onto carboxylated gold nanoparticles. Abstracts of 33^rd FEBS Congress and 11^th IUBMB Conference, Athens, Greece.

15. Chi MC, Huang HB, Lin LL. 2008. Significance of the conserved Asn345 and Asn435 residues in the catalytic activity of Bacillus kaustophilus leucine aminopeptidase as evaluated by site-directed mutagenesis. Abstracts of 33^rd FEBS Congress and 11^th IUBMB Conference, Athens, Greece.

16. Lin LL, Chen SH, Huang CC, Lo HF. 2007. Glutamic acid 219 of a truncated α-amylase from alkaliphilic and thermophilic Bacillus sp. strain TS-23 plays a critical role for its thermostability. Abstracts of Annual Scientific Meeting & Exhibition of the Australian Society for Microbiology, Adelaide, Australia.

17. Chen PJ, Chou WM, Lo HF, Lin LL. 2007. Residues Asn428 and Asn452 are important for the catalytic activity of Escherichia coli novablue g-glutamyltranspeptidase. Abstracts of Annual Scientific Meeting & Exhibition of the Australian Society for Microbiology, Adelaide, Australia.

18. Chi MC, Lin LL, Huang HB. 2007. Residues Ala348 and Gly350 are critical for the catalytic activity of Bacillus kaustophilus leucine aminopeptidase. Abstracts of Annual Scientific Meeting & Exhibition of the Australian Society for Microbiology, Adelaide, Australia.

19. Lin LL, Lia YH, Wang CH, Chen HL, Chen W. 2004. FT-Raman spectroscopic study of yam protein conformation. Abstracts of the 9^th International Conference on Raman Spectroscopy, Gold Coast, Australia.

20. Lin LL, Chyau CC, Mao JL. 1998. Production and analysis of amylolytic enzymes from thermophilic and alkaliphilic Bacilus sp. TS-23. Abstracts of the IFT Annual Meeting, Atlanta, USA.

21. Lin LL, Chyau CC, Mao JL. 1997. Cloning, sequencing and expression of an α-amylase gene from thermophilic and alkaliphilic Bacillus sp. TS-23. Abstracts of the IFT Annual Meeting, Florida, USA.

22. Tsau MR, Lin LL, Chu WS. 1995. A thermostable pullulanase from alkalophilic Bacillus sp. TS-23. Abstracts of the 7^th European Congress on Biotechnology, Nice, France.

23. Fang TY, Chu WS, Lin LL, Hsu WH. 1993. Recovery of isoamylase from Pseudomonas amyloderamosa by raw starch adsorption. Abstracts of the 6^th European Congress on Biotechnology, Firenze, Italy.

24. Lin LL, Rumbak E, Zappe H, Thomson JA, Woods DR. 1991. Cloning, sequencing and analysis of a Butyrivibrio fibrisolvens gene encoding a β-glucosidase. Abstracts of the 6^th Binary Symposium on Applied Microbiology, Stellenbosh, South Africa.

25. Tsao LS, Chen JC, Lin LL, Lee JC, Hsu WH. 1990. Cloning and characterization of theα-amylase from Streptomyces lividans TK-24. Abstracts of ASM Annual Meeting, Anaheim, California, USA.

26. Wang TT, Lin LL, Hsu WH. 1989. Cloning and expression of a Schwanniomyces occidentalis α-amylase gene in Saccharomyces cerevisiae. Abstracts of the International Conference on Biotechnology, Beijing, China.

八、研究計畫：

年度計畫名稱		補助機關
1.	87 融合蛋白質回收系統之建立 (1/3)	國科會
2.	88 融合蛋白質回收系統之建立 (2/3)	國科會
3.	89 融合蛋白質回收系統之建立 (3/3)	國科會
4.	89 嗜高溫及鹼性 Bacillus sp. TS-23 α-amylase 之突變分析	國科會
5 .	90 嗜高溫及鹼性 Bacillus sp. TS-23 α-amylase C- 端區域功能性	國科會
6 .	91 Bacillus sp. TS-23 α-amylase 必需組胺酸之鑑定	國科會
7.	91 Bacillus stearothermophilus leucine aminopeptidase之生化特性及突變分析	國科會
8.	92 Bacillus sp. TS-23 α-amylase 生澱粉結合區之選位突變	國科會
9.	92 改造 Agrobacterium tumefaciens g -胺基轉胜太以生產茶胺酸	農委會
10.	93 熱穩定白胺酸之酵素特性與突變分析 (1/3)	國科會
11.	94 熱穩定白胺酸之酵素特性與突變分析 (2/3)	國科會
12	95 熱穩定白胺酸之酵素特性與突變分析 (3/3)	國科會
13	95大腸桿菌g-麩基轉胜肽之基因表現、生物轉換應用及突變分析 (1/3)	國科會
14	96大腸桿菌g麩基轉胜肽之基因表現、生物轉換應用及突變分析 (2/3)	國科會
15	97大腸桿菌g麩基轉胜肽之基因表現、生物轉換應用及突變分析 (3/3)	國科會
16	97 Bacillus sp. strain TS-23 α-澱粉酶之熱穩定性改善及單一步驟磁性粒子純化與其配合啤酒酵母生產酒精 (1/3)	國科會
17	98 Bacillus sp. strain TS-23 α-澱粉酶之熱穩定性改善及單一步驟磁性粒子純化與其配合啤酒酵母生產酒精 (2/3)	國科會
18	99 Bacillus sp. strain TS-23 α-澱粉酶之熱穩定性改善及單一步驟磁性粒子純化與其配合啤酒酵母生產酒精 (3/3)	國科會

九、其他：

Fang, T.Y., L.L. Lin , W.S. Chu, and W.H. Hsu. Raw starch adsorption-elution purification of isoamylase. Chinese patent 070723.
Fang, T.Y., L.L. Lin , W.S. Chu, and W.H. Hsu. Method for recovery and purification of isoamylase by adsorption on raw starch. US patent 5811277.

十、教授課目：

生物高分子、核酸化學、生物技術及高等生物化學