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Der-Yen Lee |
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Title: Assistant professor |
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Education: Ph. D. 2009 Graduate Institute of Biochemical sciences , National Taiwan University , Taiwan |
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Research Area: Biochemistry, proteomics, and metabolomics. |
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Tel: 886-5-271-7900 e-mail: deryen.lee@mail.ncyu.edu.tw |
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Research Interests:
Regulations between proteomes and metabolomes in response to oxidative stress
Redox states determine the fate of cells to differentiate, proliferate, defense, and die. An oxidative stress caused by the chemical, diamide, which induces protein thiol group oxidation. In monitoring the progress of oxidative stress alleviation, the S-thiolated proteins can be isolated by resin assisted method and detected by biotin switch techniques. Therefore, the protein redox states can be determined in the whole progress. On the other hand, the switch of metabolic pathway can also be observed by LC-ESI-MS. The replenishment of NADPH from metabolism should be important for reversing the oxidative stress that can be a model for studying more details and expansion of Warburg effect. In our preliminary results, several intermediates and the correspondent enzymes have been found to be critical for the redox and metabolism in the response of oxidative stress. The establishment of the model also helps in other situations, such as inflammation, differentiation, or infection. |
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Publications: |
1. Der-Yen Lee, Geen-Dong Chang (2015, Feb). Quantitative liquid chromatography–electrospray ionization-mass spectrometry analysis of amine containing metabolites derivatized with cyanuric chloride and methylamine isotopologues. Journal of chromatography A, 1388, 60-68.
2. Shin-Jen Lin, Der-Yen Lee, Hao-Ching Wang, Shih-Ting Kang, Pung-Pung Hwang, Guang-Hsiung Kou, Ming-Fen Huang, Geen-Dong Chang, Chu-Fang Lo (2015, Jan). White Spot Syndrome Virus Protein Kinase 1 Defeats the Host Cell’s Iron-Withholding Defense Mechanism by Interacting with Host Ferritin. Journal of Virology , 89(2), 1083-1093.
3. Yi-Ting Yang, Der-Yen Lee, Yongjie Wang, Jer-Ming Hu, Wen-Hsiung Li, Jiann Horng Leu, Geen-Dong Chang, Huei-Mien Ke, Shin-Ting Kang, Shih-Shun Lin, Guang-Hsiung Kou and Chu-Fang Lo (2014, Jul). The genome and occlusion bodies of marine Penaeus monodon nudivirus (PmNV, also known as MBV and PemoNPV) suggest that it should be assigned to a new nudivirus genus that is distinct from the terrestrial nudiviruses. BMC Genomics, 15, 628-651.
4. Mei-An Su, Yun-Tzu Huang, I-Tung Chen, Der-Yen Lee, Yun-Chieh Hsieh, Chun-Yuan Li, Tze Hann Ng, Suh-Yuen Liang, Shu-Yu Lin, Shiao-Wei Huang, Yi-An Chiang, Hon-Tsen Yu, Kay-Hooi Khoo, Geen-Dong Chang, Chu-Fang Lo, Han-Ching Wang (2014, Jun). An Invertebrate Warburg Effect: A Shrimp Virus Achieves Successful Replication by Altering the Host Metabolome via the PI3KAkt-mTOR Pathway. PLOS Pathogens, Volume 10, issue 6, 2014, e1004196.
5. Der yen Lee, Geen Dong Chang (2014, Jan). Methylglyoxal in cells elicits a negative feedback loop entailing transglutaminase 2 and glyoxalase 1. Redox Biology, Volume 2, 2014, Pages 196–205.
6. Jiun-Yan Huang, Wang-Jing Liu, Han-Ching Wang, Der-Yen Lee, Jiann-Horng Leu, Hao-Ching Wang, Mong-Hsun Tsai, Shih-Ting Kang, I-Tung Chen, GuangHsiung Kou, Geen-Dong Chang, and Chu-Fang Lo. (2012, Sep). Penaeus monodon thioredoxin restores the DNA binding activity of oxidized white spot syndrome virus IE1. ANTIOXIDANTS & REDOX SIGNALING, 17(6):914-26.
7. Wen-Hsiung Ho, Der-Yen Lee, and Geen-Dong Chang (2012, May). Proteomic Identification of a Novel Hsp90-Containing Protein-Mineral Complex Which Can Be Induced in Cells in Response to Massive Calcium Influx. Journal of Proteome Research, 11, 3160-3174.
8. Fang-Yu Lin, Ching-Wen Chang, Mei-Leng Cheong, Hsei-Chorn Chen, Der-Yen
Lee, Geen-Dong Chang, and Hungwen Chen (2011, Feb). Dual-specificity phosphatase 23 mediates GCM1 dephosphorylation and activation. NUCLEIC ACIDS RESEARCH, 39(3):848-61.
9. Der-Yen Lee and Geen-Dong Chang (2009, May). Electrolytic reduction: modification of proteins occurring in isoelectric focusing electrophoresis and in electrolytic reactions in the presence of high salts. Anal. Chem., 81, P.3957-P.3964.
10. Der Yen Lee, Geen Dong Chang. Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis. Methods in molecular biology . Methods in Molecular Biology. Apr, 2009: 536, P.23-P.33.