| Jui-Chang Chen | |
| Title： Assistant Professor | |
| Education： Ph.D., Texas A&M University | |
| Research Area： Biochemistry | |
| Tel: 886-5-271-7968
e-mail: chenjc@mail.ncyu.edu.tw | |
| Research Interests：
One of my research plans is to study the etiology of spinal muscular atrophy (SMA) and to search for effective medication for the disease. SMA is an autosomal recessive genetic disease affecting motor neuron integrity. The motor neurons influence the voluntary muscles that are used for activities such as crawling, walking, and head and neck control etc. Individuals with SMA generally do not have a functional SMN1 (survival motor neuron 1) gene that produces SMN protein required for functional motor neurons. It is estimated that approximately 50 infants are the victims of SMA annually in Taiwan. However, there is no an effective drug to cure or postpone the onset of the disease. One of my research projects is to screen drugs or chemicals that are capable of altering the splicing pattern of SMN1 gene, the isoform of its products playing a critical role on the severity of spinal muscular atrophy (SMA). The other long-term objective of my research is to understand the mechanisms involved in protein sorting at sites in the endoplasmic reticulum (ER) membrane. One of my current works is to identify and characterize the mechanisms that accomplish and regulate the co-translational translocation and integration of proteins on ER. We utilize a chemical procedure for attaching a probe to the amino group on the lysine side-chain of Lys-tRNA. The fluororecent aa-tRNA analog was incorporated into newly synthesized peptide during in vitro translation, therefore provided a unique mechanism for inserting probes into the nascent chain of proteins. Several fluorescence characteristics and techniques such as fluorescence resonance energy transfer (FRET), collisional quenching, fluorescence life-time, anisotropy (or polarity), and the sensitivity of fluorescence intensity to its environment are all used in this study. | |
| Publications：
1. Hamman, B. D., Chen, J.-C., Johnson, E. E., and Johnson, A. E. (1997). The Aqueous Pore Through the Translocon Has a Diameter of 40-60 A during Cotranslational Protein Translocation at the ER Membrane, Cell 89, 535-544.
2. Li, L.-A., Chiang, E., F.-L., Chen, J.-C., Hsu, N.-C., Chen, Y.-C., and Chung, B.-c. (1999). Function of steroidogenic Factor 1 Domains in Nuclear Localization, Transactivation, and Interaction with Transcription Factor TFIIB and c-Jun, Molecular Endocrinology 13, 1588-1598.
3. Johnson, A. E, Chen, J,-C, Flanagan, J. J, Miao, Y, Shao, Y, Lin, J, and Bock, P. E. (2001). Structure, Function, and Regulation of Free and Membrane-bound Ribosomes: The View from their Substrates and Products. Cold Spring Harbor Symposia on Quantitative Biology 66, 531-541.
4. . Hou, M.-F., Chen, S.-T., Chen, J.-C., Yen, K.-T., Lee, T.-P., Chen, C.-M., Shih, M.-C., and Chang, J.-G. (2002) The correlation between CpG methylation and protein expression of P16 in oral squamous cell carcinomas. Int. J. Mol. Med. 10, 551-4.
5. Yeh, K.-T., Shih, M.-C., Lin, T.-H., Chen, J.-C., Chang, J.-Y., Kao, C.-F., Lin, K.-L., and Chang, J.-G. (2002) The correlation between CpG methylation on promoter and protein expression of E-cadherin in oral squamous cell carcinoma. Anticancer Res., 22, 3971-3975.
6. Chen ST, Chen JC, (co-first authorship) Hou MF, Yeh KT, Lee TP, Chen CM, Shih MC, Chang JG. 2003. Sulfotransferase 1A1 is a risk factor for breast cancer in young women. Mid Taiwan Journal of Medicine 8: 56-65.
7. Yeh KT, Chen JC, (co-first authorship) Chen CM, Wang YF, Lee TP, Chang JG. 2003. CYP3A5*1 is an inhibitory factor for lung cancer in Taiwanese. Kaohsiung Journal of Medical Science. 19(5): 201-207.
8. Peng, C.-T., Chen, J.-C., Yeh, K.-T., Wang, Y.-F., Hou, M.-F., Lee, T.-P., Shih, M.-C., Chang, J.-Y. and Chang, J.-G. (2003). The relationship among the polymorphisms of SULT1A1, 1A2 and different types of cancers in Taiwanese. Int. J. Mol. Med., 11, 85-89.
9. Yeh, K.-T-, Chang J.-G., Lin, T.-H., Wang, Y.-F-, Tien, N., Chang, J.-Y., Chen, J.-C., and Shih, M.-C. (2003) Epigenetic changes of tumor suppressor genes, P15, P16, VHL and P53 in oral cancer. Oncol Rep., 10, 659-663.
10. Chan, T.-F., Su, T.-H., Yeh, K.-T., Chang, J.-Y., Lin, T.-H., Chen, J.-C., Yuang, S.-S., Chang, J.-G. (2003). Mutational, epigenetic and expressional analyses of caveolin-1 gene in cervical cancers. Int. J. Oncol., 23, 599-604.
11. Su, T.-H., Chang, J.-G., Yeh, K.-T., Lin, T.-H., Lee, T.-P., Chen, J.-C., Lin, C.-C. (2003). Mutation analysis of CTNNB1 (beta-catenin) and AXIN1, the components of Wnt pathway, in cervical carcinomas. Oncol Rep., 10, 1195-1200.
12. Chen, J.-C.,. Flanagan, J.J. (co-first authorship), Miao, Y., Shao, Y., Lin, J, Bock, P. E and Johnson, A.E. (2003). SRP binds to ribosome-bound signal sequences with fluorescence-detected subnanomolar affinity that does not diminish as the nascent chain lengthens. J. Biol. Chem., 278, 18628-18637.
13. Shih MC, Peck K, Chan WL, Chu YP, Chen J.-C, Tsai CH, Chang JG.SARS-CoV infection was from at least two origins in the Taiwan area. Intervirology. 2005, 48, 124-132.
14. Yeh KT, Yang MY, Liu TC, Chen J-C, Chan WL, Lin SF, Chang JG. Abnormal expression of period 1 (PER1) in endometrial carcinoma. J Pathol. 2005, 206, 111-20.
15. Mu-Chin Shih1, Kun-Tu Yeh, Kai-Ping Tang1, Jui-Chang Chen, Jan-Gowth Chang. Promoter methylation in circadian genes of endometrial cancers detected by methylation-specific PCR. molecular carcinogenesis. 2006, 45, 732-740.
16. Lo HF, Su JY, Chen HL, Chen JC, Lin LL. Biophysical studies of an NAD(P)-dependent aldehyde dehydrogenase from Bacillus licheniformis. European biophysics. 2011, 40, 1131-1142.
17. Chin-Ming Tsai, Ming-Sheng Hsu, Jui-Chang Chen,* and Cheng-Liang Huang*. Mechanistic Study of Shape Evolution of Silver Nanoprisms in The Presence of KSCN. the journal of physical chemistry. 2012, 116, 461-467.
18. Yan-Hung Chen, Meng-Chun Chi, Tzu-Fan Wang, Jui-chang Chen and Rong-Liu Lin. Preparation of Magnetic Nanoparticles and Their Use for Immobilization of C-Terminally Lysine-Tagged Bacillus sp. TS-23 α-Amylase. Applied Biochem Biotechnol. 2012, 166, 1711-1722.
19. S-W Lee, S-H Chang, Y-S Lai, C-C Lin, C-M Tsai, Yao-Chang Lee, Jui-Chang Chen*, Cheng-Liang Huang*. Effect of Temperature on the Growth of Silver Nanoparticles Using Plasmon-Mediated Method under the Irradiation of Green LEDs. Materials. 2014, 7, 7781-7798.
20. Jan-Ying Chen, Jui-Chang Chen, Wen-Der Wang. Detection and characterization of fish secretory fluorescence substances. J. Agric. For. (NCYU). 2014, 11, 45-60
21. You-yu Syu, Jui-Hung Hung, Jui-Chang Chen, Huey-wen Chuang*. Impacts of size and shape of silver nanoparticles on Arabidopsis plant growth and gene expression. Plant Physiology and Biochemistry. 2014, 83, 57-64
22. Tsai-Chien Huang, Cheng-Liang Huang, Hung-Yu Kuo and Jui-Chang Chen*. The Association Rate of Proteins with Silver Nanoparticles is Affected by the Location of Cysteines Rather than its Number in Proteins. Chemistry (The Chinese Chemical Society, Taipei). 2015, 73, 313-322
23. J‑S JIN , L‑F LIN , JUI‑CHANG CHEN , C‑C HUANG , J‑H SHEU , WENLUNG CHEN,T‑Y TSAO and CHIH‑WEI HSU. Increased cyclin T1 expression as a favorable prognostic factor in treating gastric adenocarcinoma. ONCOLOGY LETTERS. 2015, 10, 3712-3718
24. Wen-Hau Tsai, Cheng-Liang Huang and Jui-Chang Chen*. A More Sensitive Protein-Based Method to Synthesize Fluorescent Gold Nanoclusters. Chemistry (The Chinese Chemical Society, Taipei). 2015, 73, 217-224 | |